BIOLOGY-TEXT
BIOLOGY-TEXT
5th Edition
ISBN: 9781260169621
Author: BROOKER
Publisher: MCG
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Chapter 1, Problem 2COQ
Summary Introduction

To explain: The way in which the given phenomenon is related to genomes and proteomes.

Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of both the coding as well as the non-coding parts of DNA. The study of the genome is termed as genomics. Whereas, the complete set of proteins that an organism consists is known as proteome. Proteomics is the study of proteome on a larger scale.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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