Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 1, Problem 1.8PS
A New Biofuel. As a recent cell biology graduate, you have just been hired by a
(a) Determining which genes and enzymes are required to produce the oil.
(b) Producing large amounts of a certain enzyme for further research.
(c) Studying whether any of the cellular enzymes interact with each other.
(d) Examining the involvement of any cellular organelles in storage or secretion of the oil.
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Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in
this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which
kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters.
LIPASE
+ H,C-OH
Lipase production
Vegetable oil
Methanol
Biodiesel
Transesterification
-OH
R
HO.
Lipase
+
R2
HO.
HO,
Triglyceride
Fatty acids
Glycerol
IDENTIFICATION. HELLO, BASED ON MY PRPEVIOUS EXPERIENCE HERE, BARTLEBY EXPERTS CAN ANSWER UP TO 3 SUBPARTS. THANK YOU
Identification.
a)Carrier of the activated amino acids to the ribosomes for incorporation into the growing peptide chain.
b)Molecule that attaches to myosin and actin during muscle contraction.
Question) In the factory where you work, we can provide you with hydrolysis of lactose, glucose and galactose in whey. It took you on the task of designing a bioprocess to life. Help you in design Please write the following information that will be:
a)What is the enzyme to use?
b)A current to obtain the enzyme you will use in pure form from a suitable microorganism. Create the scheme (Parameters such as enzyme being in / extracellular, MA, pI will be evaluated).
c)What kind of an experiment set would you plan to find enzyme kinetic constants?
d)What properties would you indicate on the bottle when placing the enzyme on the market?
Chapter 1 Solutions
Becker's World of the Cell (9th Edition)
Ch. 1 - What evidence led scientists to develop the basic...Ch. 1 - Why is a high degree of antibody-antigen...Ch. 1 - You have discovered a defective gene that allows...Ch. 1 - Prob. 1.3CCCh. 1 - The Historical Strands of Cell Biology. For each...Ch. 1 - QUANTITATIVE Cell Sizes. To appreciate the...Ch. 1 - QUANTITATIVE Sizing Things Up. To appreciate the...Ch. 1 - Problem Set QUANTITATIVE Limits of Resolution Then...Ch. 1 - The Contemporary Strands of Cell Biology. For each...Ch. 1 - The Facts of Life. Each of these statements was...
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