Concept explainers
a.
To determine: The number of sheets of paper printed on one side that would be required to print out the human genome.
Introduction: The Human Genome Project was an international scientific research project to determine the base pairs that make up human DNA.
b.
To determine: The tallness of the stack of paper with the entire human genome.
Introduction: The Human Genome Project is the international, collaborative research program whose goal was the complete mapping and understanding of all the genes of human beings.
c.
To determine: What would be required among a backpack shopping cart or a semitrailer truck to haul around this stack.
Introduction: The Human Genome Project is the world's largest collaborative biological project.
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Introduction To Genetic Analysis
- Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to sequence a human genome. Suppose the length of the human genome is 3x109 bps. What is the depth (i.e., coverage) of the sequencing?arrow_forwardE. How many nucleotides would be required to generate a polypeptide that is 15 amino acids long? This requires knowing how many nucleotides of DNA code for one amino acid. F. Assuming that there are between 20,000-25,000 genes in the human genome, do you think there are 1) fewer, 2) approximately the same number, or 3) more proteins in the human genome? Explain your answer.arrow_forwardGive typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.arrow_forward
- In not more than 200 words, explain how the human genome of 3.4 Gb would be in 2.3 meters long when stretched linearly if this size is fit into a cell of minuscule proportions.arrow_forwardYou have sequenced the genome of the bacterium Salmonella typhimurium, and you are using BLAST analysis to identify similarities within the S. typhimurium genome to known proteins. You find a protein that is 100 percent identical in the bacterium Escherichia coli. When you compare nucleotide sequences of the S. typhimurium and E. coli genes, you find that their nucleotide sequences are only 87 percent identical.a. Explain this observation.b. What do these observations tell you about the merits of nucleotide- versus protein-similarity searches in identifying related genes?arrow_forwardThe human genome 3.4 Gb would be 2.3 meters long if stretched linearly.In not more than 200 words,explain how a genome of this size is fit into a cell of minuscule proportions.arrow_forward
- If a virus particle contained double-stranded B-DNA of 400,000 base pairs, A. How many complete helical turns would occur on each strand? B. How many atoms of phosphorus would be present? C. If the mole % of G in this genome is 17%, what is the mole % of A?arrow_forwardWhen performing Sanger sequencing a scientist accidentally forgot to include ddGTP in his sequencing reaction (he did include ddATP, ddCTP, and ddTTP along with all the other needed components). Which of the following statements are true? A.All DNA fragments will terminate at the first G base pair B.A color will be missing from the chromatogram. C.The sequencing primer will not contain G base pairs. D.There will be gaps in the chromatogram.arrow_forwardHow many Illumina clusters would you need to generate 15X coverage of the human genome, assuming your read lenght is 600 bp? (assume a human genome size of 3 billion bp) - 2.5 billion - 75 million - 7 million - 100 millionarrow_forward
- A diploid human cell contains approximately 6.4 billion base pairs of DNA. a. How many nucleosomes are present in such a cell? (Assume that the linker DNA encompasses 40 bp.) b. How many histone proteins are complexed with this DNA?arrow_forwardsolve a given problemarrow_forwardThe plot shows a correlation between sequence error rate and cluster density on an Illumina flow cell. From your knowledge of how Illumina sequencing works, why do you think there is this correlation?arrow_forward
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