Introduction To Genetic Analysis
Introduction To Genetic Analysis
12th Edition
ISBN: 9781319114787
Author: Anthony J.F. Griffiths, John Doebley, Catherine Peichel, David A. Wassarman
Publisher: W. H. Freeman
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Chapter 1, Problem 18P

a.

Summary Introduction

To determine: The number of sheets of paper printed on one side that would be required to print out the human genome.

Introduction: The Human Genome Project was an international scientific research project to determine the base pairs that make up human DNA.

b.

Summary Introduction

To determine: The tallness of the stack of paper with the entire human genome.

Introduction: The Human Genome Project is the international, collaborative research program whose goal was the complete mapping and understanding of all the genes of human beings.

c.

Summary Introduction

To determine: What would be required among a backpack shopping cart or a semitrailer truck to haul around this stack.

Introduction: The Human Genome Project is the world's largest collaborative biological project.

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The human genome (3.4Gb) would be 2.3 metres long if stretched linearly. In not more than 200 words, explain how a genome of this size is fit into a cell if minuscule proportions
a. What type of nucleic acid and from what species would the scientist use to begin construction of her genomic DNA library? b. From what tissue would she isolate this nucleic acid? c. What type of reagent would the scientist use to cut the genome into appropriately sized fragments? d. What size nucleic acid fragments would one aim to prepare for the library construction so as to to avoid having to screen an overwhelming number of clones? e. Into what vector would the scientist ligate her genomic DNA fragments? f. What organism would the scientist use to propagate the clones of her genomic DNA library? g. From the information given in the problem determine what probe could be used to screen the scientist's library to find her clone of interest ?
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License