Your current Vmax estimate from the Michaelis-Menten plot has the units of ?/? and was determined using 0.1 mg of ADH in a total volume of 1.25 mL. Use the Beer-Lambert equation followed by a simple conversion to express your estimated Vmax in ???? per 1 mg ADH. The molar absorptivity of NADH at 340 nm is 6220 ????∗??. Following the previous instructions, express your calculated Vmax from the Lineweaver-Burk plot in ???? per 1 mg ADH.
Your current Vmax estimate from the Michaelis-Menten plot has the units of ?/? and was determined using 0.1 mg of ADH in a total volume of 1.25 mL. Use the Beer-Lambert equation followed by a simple conversion to express your estimated Vmax in ???? per 1 mg ADH. The molar absorptivity of NADH at 340 nm is 6220 ????∗??. Following the previous instructions, express your calculated Vmax from the Lineweaver-Burk plot in ???? per 1 mg ADH.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Your current Vmax estimate from the Michaelis-Menten plot has the units of ?/? and was determined using 0.1 mg of ADH in a total volume of 1.25 mL. Use the Beer-Lambert equation followed by a simple conversion to express your estimated Vmax in ???? per 1 mg ADH. The molar absorptivity of NADH at 340 nm is 6220 ????∗??. Following the previous instructions, express your calculated Vmax from the Lineweaver-Burk plot in ???? per 1 mg ADH.
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