You would like to observe an E. coli bacterium that is 2.15 μm (micrometers) long. Because diffraction blurs an image, you would like to minimize the effects of diffraction by using a wavelength no larger than the object you are observing. For this problem, assume the wavelength is equal to the length of the bacterium. What is the energy of a photon with this wavelength? What is the energy of an electron with a de Broglie wavelength of this size? In terms of energy, which particles, photons or electrons, are the least likely to damage your sensitive biological sample? electrons? photons? they are the same
Compton effect
The incoming photons' energy must be in the range of an X-ray frequency to generate the Compton effect. The electron does not lose enough energy that reduces the wavelength of scattered photons towards the visible spectrum. As a result, with visible lights, the Compton effect is missing.
Recoil Velocity
The amount of backward thrust or force experienced by a person when he/she shoots a gun in the forward direction is called recoil velocity. This phenomenon always follows the law of conservation of linear momentum.
You would like to observe an E. coli bacterium that is 2.15 μm (micrometers) long. Because diffraction blurs an image, you would like to minimize the effects of diffraction by using a wavelength no larger than the object you are observing. For this problem, assume the wavelength is equal to the length of the bacterium.
What is the energy of a photon with this wavelength?
What is the energy of an electron with a de Broglie wavelength of this size?
In terms of energy, which particles, photons or electrons, are the least likely to damage your sensitive biological sample?
- electrons?
- photons?
- they are the same
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