you SE the target ratio of phage to bacteria is 0.1 a pfu/cfu. The bacteria are at a concentration 109 cfu/ml and the phage at a concentratic
you SE the target ratio of phage to bacteria is 0.1 a pfu/cfu. The bacteria are at a concentration 109 cfu/ml and the phage at a concentratic
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:From the extract of a flowsheet, you can see that
the target ratio of phage to bacteria is 0.1 and 0.01
pfu/cfu. The bacteria are at a concentration of 1 x
109 cfu/ml and the phage at a concentration of 1 x
1011
pfu/ml. What combination of dilution factor
and volume of the phage is needed to achieve the
target pfu/ml prior to mixing? Indicate all correct
answers.
а.
1 x 104 and 0.5ml
b.
2 x 104 and 0.1ml
С.
1 x 103 and 0.5ml
d.
1 x 10' and 0.1ml
е.
1 x 108 and 0.1ml
f.
1 x 10° and 0.5ml
g.
1 x 108 and 0.5ml
h.
2 x 103 and 0.1ml
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