you SE the target ratio of phage to bacteria is 0.1 a pfu/cfu. The bacteria are at a concentration 109 cfu/ml and the phage at a concentratic

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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From the extract of a flowsheet, you can see that
the target ratio of phage to bacteria is 0.1 and 0.01
pfu/cfu. The bacteria are at a concentration of 1 x
109 cfu/ml and the phage at a concentration of 1 x
1011
pfu/ml. What combination of dilution factor
and volume of the phage is needed to achieve the
target pfu/ml prior to mixing? Indicate all correct
answers.
а.
1 x 104 and 0.5ml
b.
2 x 104 and 0.1ml
С.
1 x 103 and 0.5ml
d.
1 x 10' and 0.1ml
е.
1 x 108 and 0.1ml
f.
1 x 10° and 0.5ml
g.
1 x 108 and 0.5ml
h.
2 x 103 and 0.1ml
Transcribed Image Text:From the extract of a flowsheet, you can see that the target ratio of phage to bacteria is 0.1 and 0.01 pfu/cfu. The bacteria are at a concentration of 1 x 109 cfu/ml and the phage at a concentration of 1 x 1011 pfu/ml. What combination of dilution factor and volume of the phage is needed to achieve the target pfu/ml prior to mixing? Indicate all correct answers. а. 1 x 104 and 0.5ml b. 2 x 104 and 0.1ml С. 1 x 103 and 0.5ml d. 1 x 10' and 0.1ml е. 1 x 108 and 0.1ml f. 1 x 10° and 0.5ml g. 1 x 108 and 0.5ml h. 2 x 103 and 0.1ml
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