You prepare several dilutions of an unknown compound. You measure the pance of each solution at 340 nm using a 1 cm cuvette (your results are listed in the table- -). What is the extinction coefficient (in (Mecm)') of the compound? (Hint: assume that of the individual values contains some degree of experimental error.) entration(µM) Absorbance at 340 nm 0.011 0.023 0.066 0.119 0.189

You prepare several dilutions of an unknown compound. You measure the pance of each solution at 340 nm using a 1 cm cuvette (your results are listed in the table- -). What is the extinction coefficient (in (Mecm)') of the compound? (Hint: assume that of the individual values contains some degree of experimental error.) entration(µM) Absorbance at 340 nm 0.011 0.023 0.066 0.119 0.189

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

You prepare several dilutions of an unknown compound. You measure the
pance of each solution at 340 nm using a 1 cm cuvette (your results are listed in the table-
-). What is the extinction coefficient (in (Mecm)') of the compound? (Hint: assume that
of the individual values contains some degree of experimental error.)
entration(µM)
Absorbance at 340 nm
0.011
0.023
0.066
0.119
0.189

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