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) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). •.. --------- -++++- ----F-F-F-

) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). •.. --------- -++++- ----F-F-F-

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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**Educational Content on Clove Oil Components and Chromatography** **3. Main Components of Clove Oil** Clove oil primarily consists of the following three compounds: - **Eugenol:** A compound with a hydroxyl group causing some polarity. - **Eugenol Acetate:** An ester formed from eugenol. - **β-Caryophyllene:** A hydrophobic compound with minimal polarity. **Analysis and Chromatography Procedures** **a) Ranking Compounds by Polarity** - **From Least Polar to Most Polar:** β-Caryophyllene, Eugenol Acetate, Eugenol **b) TLC Plate Results** - After performing Thin Layer Chromatography (TLC) using a 10% acetone in hexanes mobile phase, three distinct spots were observed: - Top spot at 5 cm - Middle spot at 2.8 cm - Use the provided Rf (retention factor) to identify each spot based on their polarity and the mobile phase. **c) Calculating Rf for the Middle Spot** - **Rf Formula:** Rf = (Distance traveled by the compound) / (Distance traveled by the solvent front) - For the middle spot: Rf = 2.8 cm / 5 cm = 0.56 **d) Effect of Increasing Mobile Phase Polarity** - Increased polarity would result in higher Rf values as more polar mobile phases interact better with polar compounds, moving them further up the TLC plate. **e) Column Chromatography Separation** - **To Elute First:** β-Caryophyllene (least polar, strong interaction with non-polar stationary phase) - **To Elute Last:** Eugenol (more polar, stronger interaction with polar mobile phase) **f) Fraction Analysis Using TLC Plates** - **Column Chromatography Fractionation:** - Four fractions analyzed: 1-4, 5-8, 9-12, 13-16 - Each TLC plate shows specific spots indicating presence of different components, based on relative positions: - Identify fractions by observing which spots align with known distances from standard TLC results. Understanding this process helps in effectively separating and identifying components in mixtures, crucial for applications in chemistry and biochemistry education.
Transcribed Image Text:**Educational Content on Clove Oil Components and Chromatography** **3. Main Components of Clove Oil** Clove oil primarily consists of the following three compounds: - **Eugenol:** A compound with a hydroxyl group causing some polarity. - **Eugenol Acetate:** An ester formed from eugenol. - **β-Caryophyllene:** A hydrophobic compound with minimal polarity. **Analysis and Chromatography Procedures** **a) Ranking Compounds by Polarity** - **From Least Polar to Most Polar:** β-Caryophyllene, Eugenol Acetate, Eugenol **b) TLC Plate Results** - After performing Thin Layer Chromatography (TLC) using a 10% acetone in hexanes mobile phase, three distinct spots were observed: - Top spot at 5 cm - Middle spot at 2.8 cm - Use the provided Rf (retention factor) to identify each spot based on their polarity and the mobile phase. **c) Calculating Rf for the Middle Spot** - **Rf Formula:** Rf = (Distance traveled by the compound) / (Distance traveled by the solvent front) - For the middle spot: Rf = 2.8 cm / 5 cm = 0.56 **d) Effect of Increasing Mobile Phase Polarity** - Increased polarity would result in higher Rf values as more polar mobile phases interact better with polar compounds, moving them further up the TLC plate. **e) Column Chromatography Separation** - **To Elute First:** β-Caryophyllene (least polar, strong interaction with non-polar stationary phase) - **To Elute Last:** Eugenol (more polar, stronger interaction with polar mobile phase) **f) Fraction Analysis Using TLC Plates** - **Column Chromatography Fractionation:** - Four fractions analyzed: 1-4, 5-8, 9-12, 13-16 - Each TLC plate shows specific spots indicating presence of different components, based on relative positions: - Identify fractions by observing which spots align with known distances from standard TLC results. Understanding this process helps in effectively separating and identifying components in mixtures, crucial for applications in chemistry and biochemistry education.
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