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You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. Use the graph of bound ligand fraction (Y) versus ligand concentration ((ligand]) to estimate the dissociation constant (K) and the association constant (K).

You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. Use the graph of bound ligand fraction (Y) versus ligand concentration ((ligand]) to estimate the dissociation constant (K) and the association constant (K).

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. 1.0 Use the graph of bound ligand fraction (Y) versus ligand concentration ([ligand) to estimate the dissociation constant (Ka) and the association constant (K,). 0.75 > 0.5 0.25 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 (ligand) (M) 0.10 Ka = K. - 10 M- incorrect Incorrect
Transcribed Image Text:You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. 1.0 Use the graph of bound ligand fraction (Y) versus ligand concentration ([ligand) to estimate the dissociation constant (Ka) and the association constant (K,). 0.75 > 0.5 0.25 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 (ligand) (M) 0.10 Ka = K. - 10 M- incorrect Incorrect
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