You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. Use the graph of bound ligand fraction (Y) versus ligand concentration ((ligand]) to estimate the dissociation constant (K) and the association constant (K).

You perform a ligand binding assay using a newly isolated protein with its natural ligand and plot the resulting data. Use the graph of bound ligand fraction (Y) versus ligand concentration ((ligand]) to estimate the dissociation constant (K) and the association constant (K).

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

You perform a ligand binding assay using a newly isolated
protein with its natural ligand and plot the resulting data.
1.0
Use the graph of bound ligand fraction (Y) versus ligand
concentration ([ligand) to estimate the dissociation constant
(Ka) and the association constant (K,).
0.75
> 0.5
0.25
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
(ligand) (M)
0.10
Ka =
K. -
10
M-
incorrect
Incorrect

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