Please answer this question. You obtained the following raw data when setting up a Bradford standard curve:BSA (mg/ml)OFN M↑D ON Ø (02345QUESTION 167810Absorbancy595nm0.2250.3100.4200.5100.6100.7200.8100.9150.9500.9800.990AAfter blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.652 wasobtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

The Biuret test is a chemical test that is used to determine the presence of a peptide bond. In the…

Answered: You obtained the following raw data…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter13: Dimensional Analysis/units Conversion

Section: Chapter Questions

Problem 2.4P

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You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA (mg/ml) 595nm 0.225 1 0.310 2 0.420 0.510 4 0.610 0.720 6 0.810 0.915 8 0.950 0.980 10 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.594 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 10 0.550 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
write a detail description based on the table
Draw the Mchelis Menton plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot.
Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.
write a short description based on the table
Viscosity measurements were done on native form of Protein XYZ and on 4 denaturants. The crude Protein XYZ was diluted to produce 5% w/v. Using the Ostwald viscometer, the following data were obtained: time, sec time, sec time, sec time, sec Denaturant Blank (Native) Native Blank (Denaturant) Denatured pH= 2.00 70 95 70 101 Temp (90 °C) 70 98 70 150 0.1M Urea 72 97 88 110 DTT 71 96 90 170 a. Calculate and tabulate the reduced viscosities (in mL/g) of the native and denatured Protein XYZ. b. Determine two most effective denaturants. c. What can be inferred with the two most effective denaturants? Dont reject if you dont know the answer!
What rate in mL/hr of Isosource VHN would provide 1.6 g protein/kg/day if run for 24 hours for a patient weighing 68 kg? Round to the nearest whole number, do not enter units EN Product Кcal Protein /mL g/L General Formulations Jevity 1.5 1.5 63.4 Isosource VHN 1 62 TwoCal HN 2 83 Renal Formulations Nepro 1.8 81 Renalcal 2 34.4 Supplena 1.8 45 Glucose Control Formulations Glucerna 1 41 Choice DM 1 45
The glucose content of samples can be determined using Nelson't test. The equation of the line for the standard curve preapred for varying concentrations of glucose is y = 3.0x + 0.2. Based from this information, determine the glucose concentration (in M) of a strawberry preserve having an absorbance reading of 1.35.
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation

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