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You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.652 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.652 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) OFN M↑D ON Ø ( 0 2 3 4 5 QUESTION 1 6 7 8 10 Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 A After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.652 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
Transcribed Image Text:You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) OFN M↑D ON Ø ( 0 2 3 4 5 QUESTION 1 6 7 8 10 Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 A After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.652 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
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