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You have successfully isolated Enzyme 1 (32500 Da [g/mol]) and Enzyme 2 (65000 Da) using size- exclusion (gel filtration) chromatography. However, when analyzing the purity of your pure fractions using SDS poly acrylamide gel electrophoresis Enzyme 1 and Enzyme 2 appeared to give the same band pattern. Explain these results based on the theory behind the two individual techniques. Amount of solute Volume of effluent Ladder Enzyme 1 Enzyme 2

You have successfully isolated Enzyme 1 (32500 Da [g/mol]) and Enzyme 2 (65000 Da) using size- exclusion (gel filtration) chromatography. However, when analyzing the purity of your pure fractions using SDS poly acrylamide gel electrophoresis Enzyme 1 and Enzyme 2 appeared to give the same band pattern. Explain these results based on the theory behind the two individual techniques. Amount of solute Volume of effluent Ladder Enzyme 1 Enzyme 2

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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You have successfully isolated Enzyme 1 (32500 Da [g/mol]) and Enzyme 2 (65000 Da) using size- exclusion (gel filtration) chromatography. However, when analyzing the purity of your pure fractions using SDS poly acrylamide gel electrophoresis Enzyme 1 and Enzyme 2 appeared to give the same band pattern. Explain these results based on the theory behind the two individual techniques. Amount of solute Volume of effluent Ladder Enzyme 1 Enzyme 2
Transcribed Image Text:You have successfully isolated Enzyme 1 (32500 Da [g/mol]) and Enzyme 2 (65000 Da) using size- exclusion (gel filtration) chromatography. However, when analyzing the purity of your pure fractions using SDS poly acrylamide gel electrophoresis Enzyme 1 and Enzyme 2 appeared to give the same band pattern. Explain these results based on the theory behind the two individual techniques. Amount of solute Volume of effluent Ladder Enzyme 1 Enzyme 2
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