You have a 11.5 mg sample of blood that contains various proteins. Hemoglobin is the only protein in the sample containing Fe. Hemoglobin contains 3.83% by mass of a compound called heme (C₃₄H₃₂FeN₄O₄, MW = 616.49 g/mol). You find that your 11.5 mg blood sample contains 27.1 micrograms of Fe. What is the mass percent of hemoglobin in your protein sample?

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.