You have 500ml of a bacterial culture (cell density 1x10^6 cells/ml). How would you want to prepare 5 ml of a cell suspension with a cell density of 1x10^7 cells/ml? A. Centrifuge 100 ml of the stock B. Centrifuge 500ml of the stock C. Add 50 ml media D. Add 10 ml media E. Add 500 ml media F. Discard supernatant G. Centrifuge 50 ml of the stock H. Add 100 ml media 1. Centrifuge 10 ml of the stock J. Dissolve pellet in 5 ml media K. Dissolve pellet in 50 ml media L. Dissolve pellet in 10 ml media M. Dissolve pellet in 100 ml media
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Step by step
Solved in 4 steps
