You design an experiment to explore the effects of enzyme concentration on the rate of glucose (product) production. In the experiment, you prepare 5 tubes with equal amounts of water and substrate but altered the amount of enzyme. Tube #1 did not have enzyme, Tube #2 had 100 mg of enzyme, Tube #3 had 200 mg of enzyme, Tube #4 had 300 mg of enzyme, Tube #5 had 400 mg of enzyme. The results are summarized in the graph. Select all that apply.    Given enough time all tubes (#1-5) would reach 1100 mg/dL.   The substrate in tube #1 was denatured at 40 minutes.    The fastest rate of reaction was in Tube #4.   Adding more enzyme to Tube #5 at 40 minutes would produce more product.    Tube #4 and #5 have run out of substrate at 40 minutes.

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You design an experiment to explore the effects of enzyme concentration on the rate of glucose (product) production. In the experiment, you prepare 5 tubes with equal amounts of water and substrate but altered the amount of enzyme. Tube #1 did not have enzyme, Tube #2 had 100 mg of enzyme, Tube #3 had 200 mg of enzyme, Tube #4 had 300 mg of enzyme, Tube #5 had 400 mg of enzyme. The results are summarized in the graph.

Select all that apply. 

 

Given enough time all tubes (#1-5) would reach 1100 mg/dL.

 

The substrate in tube #1 was denatured at 40 minutes. 

 

The fastest rate of reaction was in Tube #4.

 

Adding more enzyme to Tube #5 at 40 minutes would produce more product. 

 

Tube #4 and #5 have run out of substrate at 40 minutes.

 

**Glucose Concentration Over Time in Different Test Tubes**

This graph displays the change in glucose concentration in mg/dL over a period of 60 minutes for five different test tubes. The x-axis represents time in minutes, while the y-axis indicates the amount of glucose present in mg/dL.

**Data Lines Explained:**

- **Tube #1 (Blue Solid Line with Circles):** This line shows no increase, remaining constant at 0 mg/dL for the entire 60 minutes.
- **Tube #2 (Orange Solid Line with Diamonds):** This line exhibits a gradual increase, reaching approximately 300 mg/dL by 60 minutes.
- **Tube #3 (Gray Solid Line with Crosses):** This line indicates a steady incline, culminating at around 900 mg/dL by the end of the observation period.
- **Tube #4 (Yellow Dotted Line with Circles):** This line rapidly rises and levels off just above 1000 mg/dL shortly after 30 minutes.
- **Tube #5 (Blue Dashed Line with Triangles):** This line follows a similar trajectory to Tube #4, peaking just above 1000 mg/dL by the 30-minute mark and maintaining that level.

This data suggests varying rates of glucose formation or consumption among the different samples as tested over a one-hour timeframe.
Transcribed Image Text:**Glucose Concentration Over Time in Different Test Tubes** This graph displays the change in glucose concentration in mg/dL over a period of 60 minutes for five different test tubes. The x-axis represents time in minutes, while the y-axis indicates the amount of glucose present in mg/dL. **Data Lines Explained:** - **Tube #1 (Blue Solid Line with Circles):** This line shows no increase, remaining constant at 0 mg/dL for the entire 60 minutes. - **Tube #2 (Orange Solid Line with Diamonds):** This line exhibits a gradual increase, reaching approximately 300 mg/dL by 60 minutes. - **Tube #3 (Gray Solid Line with Crosses):** This line indicates a steady incline, culminating at around 900 mg/dL by the end of the observation period. - **Tube #4 (Yellow Dotted Line with Circles):** This line rapidly rises and levels off just above 1000 mg/dL shortly after 30 minutes. - **Tube #5 (Blue Dashed Line with Triangles):** This line follows a similar trajectory to Tube #4, peaking just above 1000 mg/dL by the 30-minute mark and maintaining that level. This data suggests varying rates of glucose formation or consumption among the different samples as tested over a one-hour timeframe.
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