You are working in the biochemistry lab and your instructor asks you to perform the following procedure: 1. Make up 50 mL of a 0.100 M buffer solution at your assigned pH. 2. Add 9.50 mL of your buffer solution to 0.500 mL of 1.25 mM p-nitrophenol. 3. Take an absorbance spectrum from 360-500 nm using pure buffer solution as a blank or reference. What is the final concentration of p-nitrophenol after it is diluted in the buffer solution? Express the answer in units of mM. Note: Only include the numerical value in your answer and use 3 significant figures. Do not include units in your answer. Answer: 1.05 x

You are working in the biochemistry lab and your instructor asks you to perform the following procedure: 1. Make up 50 mL of a 0.100 M buffer solution at your assigned pH. 2. Add 9.50 mL of your buffer solution to 0.500 mL of 1.25 mM p-nitrophenol. 3. Take an absorbance spectrum from 360-500 nm using pure buffer solution as a blank or reference. What is the final concentration of p-nitrophenol after it is diluted in the buffer solution? Express the answer in units of mM. Note: Only include the numerical value in your answer and use 3 significant figures. Do not include units in your answer. Answer: 1.05 x

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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