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you are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell.

you are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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you are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell.

EXERCISE 4 Use of the Microscope 2. Use the coarse adjustment knob to bring the threads into focus. Find the area where the threads overlap. 42 3. Rotate the nosepiece to select the low-power objective lens. 4. Use the fine adjustment knob to focus through the overlapping threads. After determining which thread is on top, which is in the middle, and which is on the bottom, write your observations in the space provided. Color of top thread Color of middle thread Color of bottom thread. 4 Relationship Between Magnification and Field Diameter At scanning magnification, the diameter of the field of view is large and most of the slide specimen is visible. As magnifica- tion increases, the field diameter decreases, because at higher power the objective lens is closer to the slide and magnifies a smaller area. Figure 4.5 reviews the relationship between mag- nification and field diameter. Field diameter at scanning and low magnifications can be measured using millimeter graph paper glued to a micro- scope slide. By aligning a vertical marking on the paper with the edge of the field and then counting the number of mil- limeter (mm) squares across the field, you can determine the diameter (Figure 4.6). Knowing the diameter of the field of view enables you to estimate the actual size of an object. For example, if the field diameter is 4 mm and an object occupies one-half of the field, the object is approximately 2 mm wide. Figure 4.5 Magnification and Field Diameter Each circle on the slide illustrates the field diameter for a particular magnification; the corresponding circle outside the slide represents that magnification. Scanning power 40X Low power 100X High power 400X Figure 4.6 Calculation of Field Diameter Using Millimeter Graph Paper In this sample, the field is approximately 3.5 mm in diameter. 1 Field Diameter 2 3 Study Tip Field Diameter You can demonstrate the relationship between magnification and field diameter by curling your fingers until the thumb of each hand overlaps the index and middle fingers of the same hand. The space enclosed by the curled fingers of each hand forms the barrel of a "lens." Place these two "lenses" to your eyes, and, while sitting up straight in your chair, look at this page. Notice that you can see the entire page with this "scanning lens." Now slowly bend forward until the "lenses" are just a few inches way from the page. In this "high- magnification" view, the field of view is much smaller, and you can see only part of the page. Once you know the field diameter for one magnification -we call this lens A in the following formula-you can calcu- late the field diameter for other magnifications (lens B) using the formula shown below: Field diameter of lens B Field diameter of lens A X total magnification of lens A Total magnification of lens B QuickCheck Question 4.1 What happens to the field diameter as magnification increases?
Transcribed Image Text:EXERCISE 4 Use of the Microscope 2. Use the coarse adjustment knob to bring the threads into focus. Find the area where the threads overlap. 42 3. Rotate the nosepiece to select the low-power objective lens. 4. Use the fine adjustment knob to focus through the overlapping threads. After determining which thread is on top, which is in the middle, and which is on the bottom, write your observations in the space provided. Color of top thread Color of middle thread Color of bottom thread. 4 Relationship Between Magnification and Field Diameter At scanning magnification, the diameter of the field of view is large and most of the slide specimen is visible. As magnifica- tion increases, the field diameter decreases, because at higher power the objective lens is closer to the slide and magnifies a smaller area. Figure 4.5 reviews the relationship between mag- nification and field diameter. Field diameter at scanning and low magnifications can be measured using millimeter graph paper glued to a micro- scope slide. By aligning a vertical marking on the paper with the edge of the field and then counting the number of mil- limeter (mm) squares across the field, you can determine the diameter (Figure 4.6). Knowing the diameter of the field of view enables you to estimate the actual size of an object. For example, if the field diameter is 4 mm and an object occupies one-half of the field, the object is approximately 2 mm wide. Figure 4.5 Magnification and Field Diameter Each circle on the slide illustrates the field diameter for a particular magnification; the corresponding circle outside the slide represents that magnification. Scanning power 40X Low power 100X High power 400X Figure 4.6 Calculation of Field Diameter Using Millimeter Graph Paper In this sample, the field is approximately 3.5 mm in diameter. 1 Field Diameter 2 3 Study Tip Field Diameter You can demonstrate the relationship between magnification and field diameter by curling your fingers until the thumb of each hand overlaps the index and middle fingers of the same hand. The space enclosed by the curled fingers of each hand forms the barrel of a "lens." Place these two "lenses" to your eyes, and, while sitting up straight in your chair, look at this page. Notice that you can see the entire page with this "scanning lens." Now slowly bend forward until the "lenses" are just a few inches way from the page. In this "high- magnification" view, the field of view is much smaller, and you can see only part of the page. Once you know the field diameter for one magnification -we call this lens A in the following formula-you can calcu- late the field diameter for other magnifications (lens B) using the formula shown below: Field diameter of lens B Field diameter of lens A X total magnification of lens A Total magnification of lens B QuickCheck Question 4.1 What happens to the field diameter as magnification increases?
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