You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 6.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.4 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species in your solution. Show your calculations.
You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 6.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.4 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species in your solution. Show your calculations.
Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter16: Reactions Between Acids And Bases
Section: Chapter Questions
Problem 16.83QE
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You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 6.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.4 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species in your solution. Show your calculations.
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