You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.06. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 5.91. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH3 H₂O 0 CHỊCH NGO CH₂--0- CH,—C−O−CH,—CH,—*N–CH, → HO–CH,—CH,−*N–CH, + CH,-C−O + H* CH3 i Acetylcholine Choline CH₂ CH3 Acetate

You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.06. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 5.91. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH3 H₂O 0 CHỊCH NGO CH₂--0- CH,—C−O−CH,—CH,—N–CH, → HO–CH,—CH,−N–CH, + CH,-C−O + H* CH3 i Acetylcholine Choline CH₂ CH3 Acetate

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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