You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.6 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species
You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.6 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.6 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species in your solution. Show your calculations.
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