Write out the mechanism for transimination, the reaction of an amino acid with enzyme-bound PLP to give the amino acid-PLP imine adduct and the free Lys amino group of the enzyme. Show how general acid and base catalytic residues in the enzyme would catalyze this reaction
Write out the mechanism for transimination, the reaction of an amino acid with enzyme-bound PLP to give the amino acid-PLP imine adduct and the free Lys amino group of the enzyme. Show how general acid and base catalytic residues in the enzyme would catalyze this reaction
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Write out the mechanism for transimination, the reaction of an amino acid with enzyme-bound
PLP to give the amino acid-PLP imine adduct and the free Lys amino group of the enzyme.
Show how general acid and base catalytic residues in the enzyme would catalyze this reaction
Expert Solution
Step 1: Introduction
The transimination reaction, also known as the Schiff base formation,involving the transfer of a primary amine group from one amino acid to another, often catalyzed by enzymes. Pyridoxal 5'-phosphate (PLP) is a cofactor that plays an important role in this process. The mechanism typically involves the formation of an amino acid-PLP imine adduct and the release of the free amino group of the enzyme.
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