Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Write a short discussion based on the graph and calculate mean growth rate and mean generation time
Transcribed Image Text:Procedure
50 of
ampicillin to the
Add
3 ml
Top up to 50ml.
- Add fresh LB -
both
Mix
Sml
Take
overnight culture mixture
analyse
to
the
mixture
with 45 ml LB
broth
ODG00
The mixture is then
Measure every 15
mins. Stop until
75 mins.
Withdraw 3ml mixture to
-analyse (O0600)record as
zero time point.
Overnight culture
shaked
incubated at
(E-coli LB borth)
incubator at 37°c 200rpm
Mix
overnight culture
Sml
Take 3 ml
Add 5o ul of
ampicillin to the
mixture
mixture
to
No ampicilin
B (control) with 45 ml LB
broth
analyse
OD600
the
Measure every 15
mins. Stop until
75 mins.
The mixture is then
- incubated at
Withdrew 3ml mixture to
shaked analyse (OD600jrecord as
zero time-point.
incubator at 37c 200rpm
Graph of Absorbance vs Time(mins)
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
10
20
30
40
50
60
70
80
Time (mins)
(A)
(B)
OD 600
- Group 3(Ampicilin)
-Group 3(Control)
(minutes
Ampicillin
Control
0.121
0.133
15
0.167
0.168
30
0.053
0.248
45
0.018
0.339
60
0.053
0.509
75
0.053
0.676
Absorbance(60onm)
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