Refer to the succeeding diagram of two DNA samples (A, B) with the specific restriction enzyme(RE) recognition sites marked with arrows. A spontaneous mutation (insertion) occurred in DNAsample A and introduced an additional restriction site.RE siteRE site RE siteRE site↓↓↓ARE siteRE siteRE site↓BDesign two (2) DNA probes to be used in RFLP analysis involving these two DNA samples.Draw/illustrate the region(s) in the DNA molecule which you will use as reference(homologous) sequences in the design of the probes, THAT -a. Will reveal RFLP polymorphism between the DNA samples after probedetection/visualization; andb. Will not reveal RFLP polymorphism between the DNA samplesc. Illustrate and briefly explain the expected result from this RFLP marker analysis.(Assume that the DNA probe is labelled with 32P radioactive isotope).) [answer inmaximum 5 sentences ONLY]

Restriction Fragment Length Polymorphism (RFLP), is a molecular biology and genetics technique used…

Answered: Will reveal RFLP polymorphism between…

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Will reveal RFLP polymorphism between the DNA samples after probe detection/visualization; and

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Refer to the succeeding diagram of two DNA samples (A, B) with the specific restriction enzyme
(RE) recognition sites marked with arrows. A spontaneous mutation (insertion) occurred in DNA
sample A and introduced an additional restriction site.
RE site
RE site RE site
RE site
↓↓
↓
A
RE site
RE site
RE site
↓
B
Design two (2) DNA probes to be used in RFLP analysis involving these two DNA samples.
Draw/illustrate the region(s) in the DNA molecule which you will use as reference
(homologous) sequences in the design of the probes, THAT -
a. Will reveal RFLP polymorphism between the DNA samples after probe
detection/visualization; and
b. Will not reveal RFLP polymorphism between the DNA samples
c. Illustrate and briefly explain the expected result from this RFLP marker analysis.
(Assume that the DNA probe is labelled with 32P radioactive isotope).) [answer in
maximum 5 sentences ONLY]

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