Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining? 2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?

Q: Define the sub coating technique

A: Tablets solid formulations that undergo several modifications in the body. So, to decrease the loss…

Q: *Pure culture* o What is the desired outcome (result) of a streak plate? o Clinically, why is it…

A: Answer: PURE CULTURE : It is the microbial culture used in microbiological laboratories to promote…

Q: write the  protocol for Hexamine silver staining, including their reagents, materials, prinicple,…

A: Grocott’s alkaline hexamine-silver solution undergoes reduction to create precipitates of silver…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: Why is it necessary to prepare both NSS and Iodine Smears?

A: Stool smear:   A stool test is a type of medical diagnostic procedure that includes gathering and…

Q: Transillumination is used clinically for the detection of:

A: Transillumination is an examination that is done with the help of a strong light shining on a…

Q: What are Romanowsky stains? Name four of these.

A: Staining is a technique used to enhance contrast in samples , generally at the microscopic level.…

Q: Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite…

A: Luminol is a chemical used in many laboratory experiments, like in biotech research lab, forensic…

Q: What are the principle and basic concepts of DIFFERENTIAL staining?

A: The simple dye is used in the technique of simple staining to highlight only the specific structures…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: Explain following this procedure during preparation of temporary slide. 1)Adding water to separate…

A: Approach to Solving the Question:To properly prepare a temporary slide for microscopic examination,…

Q: List the various staining methods, and briefly characterize each.

A: Regardless of the ability of the microscope to magnify and resolve, staining is necessary to create…

Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods

A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…

Q: What makes heat an effective sterilizing agent? 2. Why are the conditions required for sterilization…

A: Sterilization in microbiology is the process of killing or removing all forms of microbial life,…

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: Why is it important to use a sterilized loop between streaks when preparinga streak­plate?

A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: culture that contains multiple types of organisms.

A: The microbiological culture or microbial culture can be defined as the method of multiplication of…

Q: 1. Give other methods used in the screening of tannins.   2. Give six examples of reagents that…

A: Tannins are vital bioactive compounds dominating the global market today. They have various…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: What staining is used for this microspecimen? a. Methilen blu b. Toluidin blu c. Eosinophilin d.…

A: Microspecimen staining is a scientific method for coloring microscopic samples of tissue or cells.…

Q: 1. As the tech in charge of the H&E stainer today you should look at the attached image. What is…

A: H&E staining is a histological staining technique that uses two dyes: hematoxylin and eosin.…

Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…

A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…

Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…

A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…

Q: What are the Types of Differential Stains? explain with an example.

A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is…

A: spectrophotometer is an device that is used for the detection of absorbance of compounds on a…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: Discuss sabin feldman dye test.

A: A Sabin–Feldman dye test is a serologic test for toxoplasmosis diagnosis. Toxoplasma trophozoites…

Q: step by step process of Methyl Red test using a schematic diagram

A: The Methyl Red Voges Proskauer protocol is used to identify and differentiate the microbes based on…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: Why is the looping out method preferable for sputum specimen for acid fast smears?

A: Sputum is the mixture of respiratory fluid and saliva, sputum analysis or sputum test is performed…

Q: Step-by-step smear preparation of Grams stain

A: Gram staining is a laboratory technique that is used to culture bacteria and identifies two kinds of…

Q: Is there a relationship between Gram staining and sensitivity to various antibacterial compounds?…

A: Gram staining is a method to identify whether a bacteria is gram positive or gram negative based on…

Q: 4. Draw and give the procedure of harada-mori technique.

A: The Harada-Mori technique is a filter-paper culture technique was performed to increase the chances…

Q: write the  protocol for mann's methyl blue-eosin staining, including their reagents, materials,…

A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…

Q: List the Comparison of Positive and Negative Stains.

A: A light microscope is the most commonly used microscope for viewing specimens. The specimens that…

Q: Describe the staining of Acid fast negative organisms.

A: The cell and its components can be visualized properly under a microscope through the staining…