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1.) What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.
Acid fasr staining is the differential staining method which was first developed by Ziehl and Neelsen. So this method is also known as Ziehl-Neelsen staining process.
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- 1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.
- 1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain?b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.What are the principle and basic concepts of DIFFERENTIAL staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)What are the principle and basic concepts of NEGATIVE staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet endospore staining methods. (omit the rinsing steps)What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)
- a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.
