Why is E. coli not the best species to use in the lab for DNA cloning and manipulation experiments? (even though we have used this bacteria the most for this purpose!) It grows slowly It has too many chromosomes and plasmids O It is not naturally transformable O It grows at a very high temperature at which DNA is not stable

Q: If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the…

A: Primers are the short stretch of nucleotide sequences, which are used in a polymerase chain reaction…

Q: What is the last step in metagenomics? Breaking open the bacterial cells Assembling short DNA…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: What would be the DNA sequence of the new strar template? O 5'TAC GGT CTG 3' O 5'AAA GCG AAU 3' O…

A: it is simple as A pairs with T and C pairs with G. So where there is C place G nd vice versa and…

Q: When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is…

A: Gel electrophoresis is a procedure used to isolate molecules by size using this charge and can be…

Q: need help

A: Recombinant DNA technology plays a vital role in in vitro experiments for producing the organism…

Q: Joining two DNA molecules using DNA ligase. Ligation. O Adhesion. Linker Linkage.

A: DNA fragment is joined with another DNA fragment by the formation of phosphodiester bond. Enzyme…

Q: The palm domain of a DNA polymerase O contains the catalytic site of the enzyme Ograbs the incoming…

A: The majority of organisms' genetic material, deoxy ribonucleic acid (DNA), contains nucleotide…

Q: The palm domain of a DNA polymerase contains the catalytic site of the enzy Ograbs the incoming…

A: Introduction Humans and nearly all other species carry their genetic information in DNA…

Q: 9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation…

A: Process of taking up nacked DNA inside the cells is called as transformation. The methods involving…

Q: You are a scientist studying the function of a gene you find interesting. You need to produce…

A: The synthesis of proteins is essential to biological processes and has numerous applications in…

Q: Why is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it…

A: RNA polymerase is an essential enzyme that catalyzes the transcription of DNA into RNA. It is a…

Q: What special property does Taq polymerase have? It binds to each strand of DNA after the two…

A:

Q: If a person is lacking a gene that codes for a protein involved in nucleotide excision repair, then…

A: DNA can experience a variety of chemical reactions, as in any other molecule. However, since DNA is…

Q: Why is telomerase not needed in prokaryotic DNA replication? O Prokaryotes have circular DNA O…

A: The telomerase is responsible for end replication in case of eukaryotic cells which perform RNA…

Q: The way that PCR amplifi es DNA is similar to the doubling in a population of growing bacteria; a…

A: A molecular biology technique that is performed to amplify the deoxyribonucleic acid (DNA) molecules…

Q: Which of the following is a difference between a viroid and a virus? O viroids contain some genes…

A: A virus is a microscopic infectious agent that can infect living organisms such as animals, plants,…

Q: The process of transferring DNA from one bacterium to another through a bacteriophage is called O…

A: Bacteriophage are obligate intracellular viruses that infect bacteria. Phage is surrounded by a…

Q: WHAT is the subject of the figure above? WHAT is the main message of the figure? Give a BRIEF but…

A: Biotechnology is the use of our comprehension of biological processes to develop practical clinical…

Q: DNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O…

A: Phosphodiester linkages connect DNA strands, which are polymers of deoxynucleoside monophosphates.

Q: Transfer of a donor chromosome fragment by a bacteriophage is defined as

A: Ans- Transduction Transformation is a type of genetic transfer that involves a bacterium taking up…

Q: The electrical current created during gel electrophoresis forces DNA to move from the negative end…

A: Answer.  DNA or Deoxyribonucleic acid is made up of nucleotides. A nucleotide contains a phosphate…

Q: You have just completed a plasmid DNA isolation procedure, but when you measured the amount DNA you…

A: Proteins are the large biomolecules and macromolecules that comprise one or more long chains of the…

Q: You want to have E. coli take up your recombinant DNA vector you designed. What is the most likely…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: The process in which recipient bacterial cells acquire genes from cell-to-cell contact with other…

A: Transfer of genes in prokaryotes occurs through horizontal gene transfer mechanisms like…

Q: Question 19 A piece of DNA is introduced into the tetracycline resistant gene of the plasmid, which…

A: Microbiology is the study of small microorganisms that cannot be seen by the naked eye. I can only…

Q: Supercoiling of DNA A requires GTP as source of energy B is not observed in eukaryotes (c) requires…

A: Requires the action of topoisomerases. Option-C

Q: This is the set-up of our experiment. There are four plates shown: LB only (a type of nutrient…

A: Bacterial transformation is the process by which bacterial competent cells take up naked DNA. The…

Q: ..... doesn't use for gene expression at global level     Microarrays maldi tof mass…

A: Ans : maldi tof mass spectrometry

Q: PCR uses a very special DNA polymerase, tell me where it came from and why it is special to PCR.

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of template DNA.

Q: using ________ will

A: (1 )Conjugation:ExplanationConjugation is a process in bacteria where genetic material is…

Q: Please help me this question ASAP need to submit urgently

A: Answer is option b.) double stranded , circular DNA molecule.

Q: Which of the following statement(s) correctly describe(s) telomerase? Select all that apply. You may…

A: DNA replication is heterogathetic process by which a new DNA stand is synthesized on the old DNA…

Q: During transduction, how does bacterial DNA end up in a phage (virus)? The phage is equally likely…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: E.coli is replicating its chromosome. If the template DNA sequence is: 3' AAA CGC GAT 5', what would…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: How has DNA sequencing technology changed over time? O It has become cheaper but is still a lengthy…

A: DNA sequencing is the process by which the arrangement of the four chemical building blocks of the…

Q: Which one of the following is NOT an acquired resistance mechanism exhibited by bacteria to protect…

A: Introduction  Small, single-celled organisms are known as bacteria. Nearly all areas of the world…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: PCR, including Real-Time PCR which is probably the most widely used molecular technique especially…

Q: In polymerase chain reaction, which of the following is NOT a purpose of the primers you added to…

A: Ans- A Explanation- PCR is the in vitro method to amplify the genomic DNA sequence. And the PCR…

Q: You start running your gel and after a few minutes notice that the colored bands from the loading…

A: Agarose gel electrophoresis is a form of electrophoresis which is used for isolating DNA fragments…

Q: Producing Insulin Many diabetics are treated using recombinant insulin. Place the following steps…

A: The first step in the production of Recombinant insulin is the identification of the gene for the…

Q: Which of the following is NOT a type of tandem repetitive DNA?       SINE     STR…

A: SOME BASIC INFORMATION ABOUT THE GENETIC MATERIAL  DNA It stands for deoxyribo nucleic acid.  It is…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.