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Q: selective agar media for gram-positive bacteria?
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Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the blood agar plates?
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- You just finished plating your electroporatio products on your YPD-Zeocin plate and you think you did everything perfectly but you come back the folloeing week and have zero colonies. Which of the following could be the reason for the absence of colonies? A) You centrifuged your electroporated cells for 30 sec at 16,000 rpm instead of 4,000 rpm before plating them B) You added sorbitol+YPD to your cells thirty minutes after pulsing your cells C) The Zeocin had not been added to the plates when they were made. D) All of the above E) Both a and b onlyWhy go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate? Explain.
- You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseA pure culture was inoculated onto a Mueller-Hinton agar plate. The Kirby-Bauer procedure was performed. One of the drugs tested showed a large zone of inhibition but also had small colonies growing within this zone. Further testing showed that these colonies were not the results of contamination. Why would these colonies be present within this zone of inhibition?
- You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?