Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
The technician then plates 0.1mL of the last three dilutions in duplicate. Why are the last three dilutions plated, as opposed to only one dilution? Why are the dilutions plated in duplicate?
Transcribed Image Text:Dilution Scheme to Achieve 100 PFU/Plate
10 μιL
10 μι
66.66 µl
100 μL
100 μL
100 μι
1
1
1
1
1
1
100
100
15
10
10
10
Dilution #
1
3
4
6.
LB (Diluent) µl
990
990
933.34
900
900
900
Dilution Factor
102
104
1.5x105
1.5x106
1.5x107
1.5x108
PFU/0.1mL
1.5x107
1.5x105
104
103
102
10
1.5 x 1010
PFU/mL
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