Which way will negatively charged molecules migrate? in the opposite direction of the current to the negative electrode to the positive electrode
1. Which way will negatively charged molecules migrate?
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in the opposite direction of the current
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to the negative electrode
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to the positive electrode
2. What is the purpose of the buffer in the gel chamber?
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The buffer in the gel chamber acts as an electrical circuit between the electrodes and controls the pH during electrophoresis.
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To protect the gel from burning when electrified.
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To provide a medium for the samples to sink through.
3. Why do the samples need to be denser than the buffer?
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to keep the samples in the bottom of the well
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to keep the samples in the top of the well
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so that the samples move slower than the buffer
4. What substance is used to make the samples denser?
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salts dissolved in water
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buffer solution
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glycerol or sucrose
5. What is the gel made of?
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gelatin
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Agarose is a polysaccharide, generally extracted from certain red seaweed.
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microfiber mesh made up of organic
polymers
6. Name the charged
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proteins, carbohydrates, and
nucleic acids -
salts, and nonionic compounds
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nonpolar and neutral organic molecules and binary salts
7. On what basis does electrophoresis separate molecules?
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magnitude of charge but not relative size
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magnitude of charge and relative size
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relative size but not magnitude of charge
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by polarity and magnitude of charge
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