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Which of the following types of mutations are likely to result from imperfect NHEJ repair of a double-strand DNA break caused by Cas9 cleavage? Select all that apply (focusing on what happens right at the repair site, not upstream or downstream) Frameshift O Nonsense point mutation Indel Silent point mutation O Missense point mutation

Which of the following types of mutations are likely to result from imperfect NHEJ repair of a double-strand DNA break caused by Cas9 cleavage? Select all that apply (focusing on what happens right at the repair site, not upstream or downstream) Frameshift O Nonsense point mutation Indel Silent point mutation O Missense point mutation

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which of the following types of mutations are likely to result from imperfect NHEJ repair of a double-strand DNA break caused by Cas9 cleavage? Select all that apply (focusing on what happens right at the repair site, not upstream or downstream) Frameshift Nonsense point mutation Indel Silent point mutation Missense point mutation
Transcribed Image Text:Which of the following types of mutations are likely to result from imperfect NHEJ repair of a double-strand DNA break caused by Cas9 cleavage? Select all that apply (focusing on what happens right at the repair site, not upstream or downstream) Frameshift Nonsense point mutation Indel Silent point mutation Missense point mutation
You are working on developing a gene therapy method to repair (in humans) the missense mutation in the beta-globin gene that causes sickle cell anemia (SCA) using CRISPR/Cas9 with homology-directed repair. What do you need to introduce into blood stem cells from a SCA patient so make this work? A repair template containing the wild-type beta-globin gene sequence Cas9 gene Full-length beta globin mRNA A repair template containing the mutant beta-globin gene sequence Guide RNA whose target region sequence is complementary to the portion of the beta-globin gene were the SCA mutation resides
Transcribed Image Text:You are working on developing a gene therapy method to repair (in humans) the missense mutation in the beta-globin gene that causes sickle cell anemia (SCA) using CRISPR/Cas9 with homology-directed repair. What do you need to introduce into blood stem cells from a SCA patient so make this work? A repair template containing the wild-type beta-globin gene sequence Cas9 gene Full-length beta globin mRNA A repair template containing the mutant beta-globin gene sequence Guide RNA whose target region sequence is complementary to the portion of the beta-globin gene were the SCA mutation resides
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