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- Which classes of biomolecules would be best separated using normal phase chromatography? Why? steroids mono- and disaccharides flavonoids5.A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.0. The process used to join two molecules of nucleic acid together (or any two organic molecules, for that matter) is called ________________________ ___________________________ .
- A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg)Activity (units) 1. Crude extract 20,000 4,000,000 2. Precipitation (salt) 5,000 3,000,000 3. Precipitation (pH) 4,000 1,000,000 4. lon-exchange chromatography 200 800,000 5. Affinity chromatography 50 750,000 6. Size-exclusion chromatography 45 675,000 (a) From the information given in the table, calculate the specific activity of the enzyme after each purification procedure.A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg)Activity (units) 1. Crude extract 20,000 4,000,000 2. Precipitation (salt) 5,000 3,000,000 3. Precipitation (pH) 4,000 1,000,000 4. lon-exchange chromatography 200 800,000 5. Affinity chromatography 50 750,000 6. Size-exclusion chromatography 45 675,000 (a) From the information given in the table, caleulate the specific activity of the enzyme after each purification procedure. (b) Which of the purification procedures used for this enzyme is most effective? (c) Which of the purification procedures is least effective? (d) Is there any indication based on the results shown in the table that the enzyme after step 6 is now pure? (e) What else could be done to estimate the purity of the enzyme preparation?A technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hara [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit. Note: You may need to round the numbers to get the required answer. Final volume of solution: 200 ml VEENER Total buffer compound concentration: 150 mM Ratio conjugate base/weak acid: 1.58/1 2000 Concentration weak acid: 0.058 M Final pH of solution: 6.5 Buffer compound pk.: 6.3 14 13 12 11 10 9
- Are there any components found between the macromolecule test reagents (Benedict's, Iodine, Sudan and Biuret's)? Write in your own words. Do not copy and paste from google.The model I was given is picene and I need to answer this question:A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total Protein (Mg) Activity (Units) Crude Extract 20,000 4,000,000 Precipitation (Salt) 5,000 3,000,000 Precipitation (pH) 4,000 1,000,000 Ion Exchange Chromatography 200 800,000 Affinity Chromatography 50 750,000 Size-exclusion Chromatography 45 675,000 a) From the information given in the table, calculate the specific activity of the enzymeafter each purification procedure.b) Which of the purification procedures used for this enzyme is most effective (i.e., givesthe greatest relative…
- Give Detailed Solution..(no need Handwritten)Give me a clear Detailed Solution..no need handwrittenHello sir, please, I want a simple talk about this subject, I understand it means abbreviation. Polymeric Biomaterial ОВЈЕСТ: Definition, classifications and application of polymeric biomaterials. Introduction