Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
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Q: Why does the endospore retain the green stain, while the ordinary cells do not?
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Q: can you specify what are they positive for? like Positive for Xanthoproteic test (letters)**…
A: Phenols are compounds that have one or more hydroxyl groups attached to a benzene ring.…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
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Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: A В C
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Q: Explain the principle of capsule staining?
A: The staining process is performed for visualisation purpose under microscope. Actually the chemical…
Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.
A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…
Q: The capsule stain is considered a negative stain
A: Staining is a technique in microbiology so as to enhance the view by contrasting it. Stains are…
Q: What are the Types of Differential Stains? explain with an example.
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Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: Why is it necessary to perform a negative stain to visualize capsules?
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Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: What is the color of the capsule in the capsule stain?
A: The purpose of capsule stain is to differentiate the capsular part from the bacterial cell. Capsule…
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Q: What is the mordant in the spore stain?
A: A mordant is any substance, which is used as a fixative to bind the dye on the substance.
Q: Is the gram stain significant to microorganisms other than bacteria? explain
A: The Gram stain is primarily used to differentiate bacteria into two broad categories: Gram-positive…
Q: Outline in detail the procedure for celloidin embedding .
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Q: cal is utilized as the reagent known as VP-B?
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Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
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- it's so confusing, can you specify what are they positive for? like Positive for Xanthoproteic test (letters)** Negative for Xanthoproteic test. (letters)** Negative for phenol group (letters)** Has the presence of phenol group. (letters)**About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?What are the principle and basic concepts of NEGATIVE staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- What does this diagram illustrate? Complete the labels.Is it possible to perform double or triple staining using fluorescent dyes? Explain.Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.
- Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsThe capsule stain is considered a negative stainWhat is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method?