When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer.   Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.   The alcohol wash removes crystal violet and iodine from the bacteria.   Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.   Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
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When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink?

Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer.
 
Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.
 
The alcohol wash removes crystal violet and iodine from the bacteria.
 
Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.
 
Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
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