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- Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information answer: The ATP generated by oxidative respiration is used throughout the cell. The majority of ATP production occurs in the mitochondrial matrix. How do you think ATP is made accessible to enzymes in the cytosol and other organelles?Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information answer: If the inner mitochondrial membrane were rendered as permeable as the outer membrane, how would that affect oxidative phosphorylation? Which specific processes would stop and which remain?Select the correct answer : Which of the following statements best describes the chemiosmotic theory of ATP synthesis by the electron transport system? A) As the protons pass through the system, electrons are moved from the mitochondrial matrix to the intermembrane space and ATP is formed as the electrons move back to the matrix through only one phosphorylation site. B) As the electrons pass through the system, ATP is synthesized at three different phosphorylation sites. C) As the electrons pass through the system, protons are moved from the mitochondrial matrix to the intermembrane space and ATP is formed as the protons ;move back to the matrix through the three different phosphorylation sites. D) As the electrons pass through the system protons are moved from the mitochondrial matrix to the intermembrane space and ATP is formed as the protons move back to the matrix through only one phosphorylation site.
- Using the answer code on the right, indicate which form of energy production is being described: 1. takes place in the mitochondrial matrix 2. produces H2O as a by-product 3. results in a rich yield of ATP 4. takes place in the cytosol 5. processes acetyl-CoA 6. takes place in the mitochondrial innermembrane cristae 7. converts glucose into two pyruvate molecules 8. uses molecular oxygen 9. accomplished by the electron transport system and ATP synthase (a) glycolysis (b) citric acid cycle (c) oxidative phosphorylationIn the 1930s, some physicians prescribed low doses of a compound called dinitrophenol (DNP) to help patients lose weight. This unsafe method was abandoned after some patients died. DNP uncouples the chemiosmotic machinery by making the lipid bilayer of the inner mitochondrial membrane leaky to H+ . Explain how this could cause weight loss and death.As protons (H+) pass through the mitochondria inner membrane… Question 10 options: a) Protons follow their concentration gradient. b) All the potential energy contained in the proton gradient is converted into ATP. c) Some of the potential energy contained in the proton gradient is converted into ATP and some is released as heat (i.e., unusable energy). d) Protons go up in potential energy level and ∆G > 0. e) A and C
- The maintenance of a proton motive force across the inner mitochondrial membrane is crucial for continued ATP production. Surprisingly, it has been discovered that the inner membranes of certain cells contain proteins, called uncoupling proteins, that are capable of transporting protons from the intermembrane space to the mitochondrial matrix. Why would mitochondria contain transporters that essentially waste energy potential in the proton gradient?1 a) What is meant by the ATP currency exchange ratio? Why does the oxidation of mitochondrial FADH2 generate one less ATP than oxidation of mitochondrial NADH? b) If 12 H+ are moved across the inner mitochondrial membrane by NADH oxidation, and each ATP synthesized requires 3 H+ to move through ATP synthase, why are only 3 ATP molecules produced by oxidation of each NADH?"The three respiratory enzyme complexes in the mitochondrial inner membrane tend to associate with each other in ways that facilitate the correct transfer of electrons between appropriate complexes.", is true or false.
- The inner mitochondrial membrane exhibits all of the fundamental characteristics of a typical cell membrane, but it also has several unique characteristics that are closely associated with its role in oxidative phosphorylation. What are these unique characteristics? How does each contribute to the function of the inner membrane?a) In the common electron transport pathway, electrons move from reduced nicotinamide adenine dinucleotide (NADH) to the terminal electron acceptor found in eukaryotic mitochondria. Discuss the roles of mitochondria as a powerhouse in eukaryotes.The glycerol-3-phosphate shuttle can transport cytosolic NADH equivalents into the mitochondrial matrix (see Fig. 15.11c). In this shuttle, the protons and electrons are donated to FAD, which is reduced to FADH₂. These protons and electrons are subsequently donated to coenzyme Q in the electron transport chain. End of Chapter Problem 86a How much ATP is generated per mole of glucose when the glycerol-3-phosphate shuttle is used? (Tolerance is +/- 2%) ATP are generated per glucose.