Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Step 1
TLC :-
- Thin - layer chromatography is a methods, whose distinguishing experimental characteristic is a very thin sheet coating of a powdered a stationary phase on a glass, metal or plastic sheet.
- This sheet serves as stationary phase.
- The liquid mobile phase moves through stationary phase by capillary action .
- Thin layer chromatography use absorption as the separation mechanism.
- Thin layer chromatography is used to separate mixture of components.
- A compound will have different affinities for the mobile and stationary phases, and this affects the speed at which it migrates. This separation depends on affinity of compounds towards mobile phase and stationary phase.
- In TLC the aim is to obtain well separated , well defined spots.
- After separation, spots are obtained on TLP plate.
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