What reaction is taking place in the above activity?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Topic is all about enzymes

Procedure:
1. Prepare 6 test tubes and place 1 ml saliva in each.
2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to
tube 5 and 10 ml to tube 6. Mix thoroughly.
3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and
add 1 ml of 1% starch paste. Mix well and heat in a water bath with
temperature maintained at 40°C for 30 minutes.
4. Divide each of the contents of each tube into 2 and test with:
a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI
and note the color produced. Compare the intensity of color in each of the
6 tubes.
b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of
each tube and heat in a boiling water bath for 3 minutes. Note color of
precipitate.
5. Rank each tube according to decreasing reaction rate one (1) being the fastest
to hydrolyze and 6 being the slowest.
Transcribed Image Text:Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
5. What reaction is taking place in the above activity?
Transcribed Image Text:5. What reaction is taking place in the above activity?
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