What is the role of liquid chromatography in protein purification?

Q: What factor allows molecules to be separated by chromatography?

A: To find The factor that allows molecules to be separated by chromatography

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: What other approaches can be used to determine protein concentration?

A: Introduction :  Proteins are nitrogenous organic substances made up of one or more elongated chains…

Q: What is optical density (OD) and how is it measured? How is it related to the concentration of the…

A: UV–vis spectrophotometric optical density (OD) is the most commonly-used technique for estimating…

Q: a) You work as a summer student in a lab to help a postdoctoral fellow in her research. One day she…

A: Molarity = Moles/Volume in liters Since the solution volume had to be 20mL ( = 0.02 L) with a…

Q: What are some advantages of sequencing peptides by mass spectrometry rather than by Edman…

A: Edman degradation:  It is a three-stage reaction that tags and removes the N-terminal residue of a…

Q: Why was an SDS-PAGE gel used when working with a protein as opposed to an agarose gel? What dye was…

A: SDS PAGE, abbreviation of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a laboratory…

Q: Explain the methods of Protein Engineering?  Please answer at your own words.

A: Protein engineering is the change in protein structure to suit the needs. It may be used in…

Q: What are advantages and disadvantages of solid phase bioremediation technique?

A: As we know environmental pollution are rising day by day so the bioremediation is the best method to…