Short answer questionsWhat is the reaction used to break the bond linking cysteine residues and howcan one prevent it's subsequent reformation?1.In a protein at pH7 what is the charge differences resulting from acetylation,disulfide reduction, deamidation and pyroglutamic acid?2.3. How does one use HPLC/MS with ETD to determine the disulfide connectivity of anantibody drug? ?4.What is the advantage of the use of size exclusion chromatography with lowangle light scattering (LALLS) to measure aggregate in a protein product?

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What is the reaction used to break the bond linking cysteine residues and how can one prevent it's subsequent reformation? 1.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.

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