What is the reaction used to break the bond linking cysteine residues and how can one prevent it's subsequent reformation? 1.

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter3: Biological Macromolecules
Section: Chapter Questions
Problem 33CTQ: Aquaporins are proteins embedded in the plasma membrane that allow water molecules to move between...
icon
Related questions
icon
Concept explainers
Question
Short answer questions
What is the reaction used to break the bond linking cysteine residues and how
can one prevent it's subsequent reformation?
1.
In a protein at pH7 what is the charge differences resulting from acetylation,
disulfide reduction, deamidation and pyroglutamic acid?
2.
3. How does one use HPLC/MS with ETD to determine the disulfide connectivity of an
antibody drug? ?
4.
What is the advantage of the use of size exclusion chromatography with low
angle light scattering (LALLS) to measure aggregate in a protein product?
Transcribed Image Text:Short answer questions What is the reaction used to break the bond linking cysteine residues and how can one prevent it's subsequent reformation? 1. In a protein at pH7 what is the charge differences resulting from acetylation, disulfide reduction, deamidation and pyroglutamic acid? 2. 3. How does one use HPLC/MS with ETD to determine the disulfide connectivity of an antibody drug? ? 4. What is the advantage of the use of size exclusion chromatography with low angle light scattering (LALLS) to measure aggregate in a protein product?
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Microbial taxonomy
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning