What is the importance of pyrophosphate in the synthesis of nucleic acids ?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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What is the importance of pyrophosphate in the synthesis of nucleic acids ?

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Deoxyribonucleic acid (DNA) is synthesized by DNA polymerases. DNA polymerase catalysis the formation of phosphodiester bond between incoming nucleotide and 3' OH group of the terminal primer. The synthesis occur in 5' to 3' direction. Presence of all four dNTPs(deoxyribonucleoside triphosphate) is essential for synthesis.

Phosphodiester bond- It is formed by condensation between hydroxyl group of two sugars and a phosphate group.

Pyrophosphate- Two phosphate groups are joined by esterification.

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