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What is the molarity of the methylene blue stock solution? (I dont know how to calculate it based on the information that is provided)

What is the molarity of the methylene blue stock solution? (I dont know how to calculate it based on the information that is provided)

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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What is the molarity of the methylene blue stock solution? (I dont know how to calculate it based on the information that is provided)
Materials Methylene blue solution (0.26% m/v, M.W. 320g/mole) Methylene blue solution of unknown concentration 100 mL of water Test tubes Method 1. From the stock solution of methylene blue, prepare 5 mL of a methylene blue solution at a final concentration of 0.4 mM. Label this tube Nº1. Make sure to write down how this solution was prepared. 2. Add to a test tube, labelled Nº2, 1.2 mL of water and 4.8 mL of solution Nº1. 3. Add to a test tube, labelled Nº3, 3.0 mL of water and 2.5 mL of solution Nº2. 4. Add to a test tube, labelled Nº4, 1.5 mL of water and 2.0 mL of solution Nº3. 5. Add to a test tube, labelled Nº5, 1.0 mL of water and 1.6 mL of solution N°4. 6. Add to a test tube, labelled Nº6, 1.0 mL of water and 1.5 mL of solution Nº5. 7. From the stock solution of methylene blue of unknown concentration, prepare dilutions in a final volume of 5 mL of 1/4.5 and of 1/8. Label these test tubes UNK 1 and UNK 2 respectively. Make sure to write down how these dilutions were prepared. 8. Transfer 0.1 mL of each of the solutions to the wells of a 96 well plate as indicated below. 9. Obtain the absorbance readings at 550 nm.
Transcribed Image Text:Materials Methylene blue solution (0.26% m/v, M.W. 320g/mole) Methylene blue solution of unknown concentration 100 mL of water Test tubes Method 1. From the stock solution of methylene blue, prepare 5 mL of a methylene blue solution at a final concentration of 0.4 mM. Label this tube Nº1. Make sure to write down how this solution was prepared. 2. Add to a test tube, labelled Nº2, 1.2 mL of water and 4.8 mL of solution Nº1. 3. Add to a test tube, labelled Nº3, 3.0 mL of water and 2.5 mL of solution Nº2. 4. Add to a test tube, labelled Nº4, 1.5 mL of water and 2.0 mL of solution Nº3. 5. Add to a test tube, labelled Nº5, 1.0 mL of water and 1.6 mL of solution N°4. 6. Add to a test tube, labelled Nº6, 1.0 mL of water and 1.5 mL of solution Nº5. 7. From the stock solution of methylene blue of unknown concentration, prepare dilutions in a final volume of 5 mL of 1/4.5 and of 1/8. Label these test tubes UNK 1 and UNK 2 respectively. Make sure to write down how these dilutions were prepared. 8. Transfer 0.1 mL of each of the solutions to the wells of a 96 well plate as indicated below. 9. Obtain the absorbance readings at 550 nm.
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