Q: What is the difference of washing from hand disinfection?
A: Introduction:- Hand hygiene is very important for health. Hands are very important route of germs…
Q: What gram-positive bacterium can give a positive presumptive test?
A: In microbiology, the presumptive test is used to find out the presence of gas-forming lactose…
Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.
A: Protein is a nitrogenous organic macromolecule that is essential to human health. It is responsible…
Q: What concentration of agar is used in a semisolid medium for motility determination?
A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…
Q: Does a positive presumptive test mean that the water is absolutely unsafe to drink?
A: In microbiology, the presumptive test is used to find out whether gas-forming lactose fermenters…
Q: Why is glucose not the choice of carbohydrate for most differential media used in the identification…
A: Bacilli present in the intestinal tract are rod shaped bacteria.for example, enteric bacteria…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: What is the significance of heat fixing the smear?
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: Why would hydrogen peroxide be an effective disinfectant?
A: We have to answer why hydrogen peroxide can be an effective disinfectant.
Q: what is the culture media for Vibrio Cholerea?
A: ANSWER;- Culture media for Vibrio Cholera is a Laboratory Method for the Diagnosis of Vibrio…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: What temperature is considered adequate for achieving sterilization of a compost pile?
A: temperature considered for achieving sterilization of a compost pile is 135-160 F. At a…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: What are high level disinfectants? What is an example of one? What is the method of action and the…
A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: How alcohol is effective at contolling microbial growth?
A: Sterilization: Sterilization includes the complete wipeout of all microscopic life on a surface.…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Which microorganism does not contain superoxide dismutase enzymes?
A: Superoxide dismutase (SOD) is an enzyme found altogether in living cells. An enzyme could be a…
Q: Which medium is used for indole test and Coagulase Test?
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: What is the difference between a Hemin test and a Benzidine test?
A: Hemoglobin is a blood protein that is responsible for oxygen transport in the tissues. Hemoglobin…
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: What is the result of Staphylococcus epidermidis in the TSI agar test?
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…
Q: List one method of disinfection, and one method of sterilization. Label which is which.
A: Disinfection is a process by which most of the harmful microbes are killed or inactivated. However…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: What is the point of the pressure in the autoclave sterilization?
A: Autoclaves are the devices which are like pressure cookers and used in laboratories that provide a…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: write a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium…
A: The biochemical and microbial tests are performed to analyze the capability and function of the…
Q: Methyl red test does the color of the medium change to red when methyl red was added? Is it positive…
A: Biochemical method of bacterial identification is a classical method that is used for the…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…
Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…
A:
Q: What is the temperature for pasteurisation?
A: There are several methods to preserve food products and beverages or to increase their shelf life,…
Q: What microorganisms other than coliforms are likely to give positive presumptive test?
A:
Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…
A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least…
A: Introduction :- Micro-organisms are classified into different categories on the basis of the…
Q: which kind of sample gives positive result for Molish test?
A: It is a chemical test which is named after the botanist Hans Molisch. This test involves the…
Q: Is Vinegar a disinfectant?
A: It is not an EPA-registered disinfectant that means it won't kill 99.9 percent of bacteria and…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: What might have been some of the culture conditions used to isolate A. fumarolicum?
A: A.fumarolicum is an Extremophile. They are acidophilic, thermophilic and methanotropic in nature…
Q: Based on the bacterial cultivation, what are the possible organisms under its gram reaction?
A: Gram reaction in microbiology is a staining procedure that is used to differentiate bacteria based…
Q: If you suspect that your microbial culture growing in nutrient broth is contaminated, what can you…
A: The contamination refers to the growth of one or more undesired cultures in your pure sample. It can…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
What is the media used in Urease Test and expected results?
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- Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different reasons. Explain.From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?What are the mechanism of action of hydrogen peroxide, rubbing alcohol, lab disinfectant and copper sulfate? Name a standard test for the effectiveness of a disinfectant. Name the chemical agent that is used as a standard. What are the standard test organisms?