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Q: What information do learn from the results of the DNA extraction?
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What is the importance of human dna extraction and what are the properties of DNA that can be found out in cheek cell DNA extraction?
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- What are sticky ends? What is their importance in recombinant DNA technology?What are the characteristics of a high-quality DNA extract? What do you do to determine the quality of your DNA extracts?What are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?
- What are the steps in extracting DNA from a Banana using simple household materials like dishwashing liquid and cold isopropyl alcohol? Explain the principlesWhat is the application of DNA extraction in healthcare? Cite at least 3 specific examples.For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityIn gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of the electric field? The positive pole? Why are these attractions and repulsions important to the process?What are the barriers to getting DNA out of a cell (in particular a plant cell)?
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.Why was it necessary to mash the strawberries extensively with your hands? (hint: you wouldn’t have to do this step with an animal cells). Why do we treat the cells with soap when conducting DNA extraction? And why do we add salt when doing the DNA extraction?What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.