What is the E-value of the sequence, what does your E-value reflect and what’s the importance of an E-value?

Q: Why are the correct primer sequences essential for successful amplification?

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: What is a non replicative conservative transposon?

A: Transposons are the DNA segment that is known to move from one location to another within a genome.…

Q: Why is it preferable for the delta G of the hairpin loop to be more positive in Primer desgin?

A: Hairpin loops are a common secondary structure that can form in single-stranded DNA or RNA…

Q: List three rules for designing primers?

A: A primer is short ,single strand DNA sequence used in the polymerase chain reaction. Primers are…

Q: What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR)…

A: Genome editing can be defined as the way of of making  the specific changes to the DNA of a cell or…

Q: What is meant by Genetic Information NondiscriminationAct (GINA) ?

A: Genetics can be defined as the branch of biology which is concerned with the study of genes, genetic…

Q: What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in…

A: To transfer set of desired artificial or natural genes into target organisms employs the need of any…

Q: What is reverse tandem duplication?

A: Duplication is a type of mutation, which involves the production of one or more copies of a gene or…

Q: How are the repeated sequences generated?

A: Introduction Repeated sequences are patterns of nucleic acids (DNA/RNA) that occur at multiple sites…

Q: How would you detect the SNP?

A: SNP  -Single nucleotide polymorphism         A single nucleotide replaced with other nucleotides in…

Q: What is expressed sequence tag (EST)?

A: The sequencing of the genome from different organisms has been performed for many years due to many…

Q: What do the spacers within the CRISPR region correspond to?

A: CRISPR that is clustered regularly interspaced short palindromic repeats. It is found in the…

Q: What is a CT value and what is its importance in interpreting gene expression?

A: Using technology in biology has changed the way we understand living things and has opened up new…

Q: In a cotransduction experiment involving P1, the cotransductionfrequency was 0.53. How far apart are…

A: Transduction is the process of the transferring foreign DNA into the host cell by viral vector. T4…

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: Why did we obtain the sequence from the forward primers separate from the reverse primer?

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: What is a retromer?

A: Cell surface receptor are the receptor that are embedded in the plasma membrane of cells. They act…

Q: What is the difference of intragenic and intergenic regions? Please explain with drawing.

A: In the intricate tapestry of the genome, the terms "intragenic" and "intergenic" encapsulate…

Q: When performing contract relax (CR) stretching, how many times can the sequences be repeated?

A: Contract-Relax (CR) stretching is a widely employed technique in flexibility training that involves…

Q: What are ‘Selectable marker’? What is their use in genetic engineering?

A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…

Q: There’s been a murder and four possible suspects are being questioned. A blood sample has been found…

A: SSRs ( simple sequence repeats ) or micro satellite sequences in DNA play a major role as molecular…

Q: Define deletion-insertion polymorphisms (DIPs)

A: Insertion is a mutation in which one or more nucleotide base pairs are added into a DNA sequence.…

Q: How were the specific sequences of triplet codes determined experimentally?

A: The DNA genetic code comprises a set of rules and instructions to obtain proteins and molecules…

Q: What causes trinucleotide repeat expansion?

A: Trinucleotide repeat expansion refers to the phenomenon where trinucleotide sequences in certain…

Q: What are primer dimers? How are they formed?

A: PCR stands for polymerase chain reaction. It is used to amplify the region of gene.

Q: What are Exceptional genetic codes?

A: A genetic code translates the genetic information encoded within the deoxyribonucleic acid (DNA) or…

Q: Transposons in bacterial genomes can carry genes,including those conferring ________ resistance

A: The transposable elements are known as stretches of DNA molecules that can jump from one place to…

Q: What are Watson-Crick base pairs? Why are they important?

A: DNA or deoxyribonucleic acid is a type of biomolecule. It is a polymer of deoxyribonucleotides…

Q: Some enzymes are considered as molecular scissors. in genetic engenrring. What is the name assigned…

A: Genetic engineering is a process by which the genetic makeup of an organism can be altered. This…

Q: What different between the generated amplification product and the primer sequences in the CDS…

A: An increase in the number of copies of a gene in a genome is referred to as gene amplification. In…

Q: What is the utility of understanding the genetic basis of susceptibility to COVID-19 for medicine…

A: To date, 3p21. 31 is the only genetic region significantly associated with severe COVID-19. Having…

Q: Is there any difference between the alignments, alignment scores, identity percentages, positives…

A: Introduction :- The process of  characterizing sequences of biomolecules particularly the…

Q: What is a copy number variant in genetics?

A: Genes are the structural and functional units of heredity. They carry coded genetic information in…

Q: What does massively parallel sequencing mean?

A: