What is likely to be the problem if after you add substrate, all the wells begin to change to a deep color, including your controls? You may choose more than one Othe washing was not sufficient O the antibodies used to coat the wells are not binding the antigen O you may not have blocked the wells appropriately O the antibodies used to coat the wells are not specific to the antigen and bind many serum proteins

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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What is likely to be the problem if after you add substrate, all the wells
begin to change to a deep color, including your controls? You may choose
more than one
the washing was not sufficient
the antibodies used to coat the wells are not binding the antigen
O you may not have blocked the wells appropriately
O the antibodies used to coat the wells are not specific to the antigen and bind
many serum proteins
Transcribed Image Text:What is likely to be the problem if after you add substrate, all the wells begin to change to a deep color, including your controls? You may choose more than one the washing was not sufficient the antibodies used to coat the wells are not binding the antigen O you may not have blocked the wells appropriately O the antibodies used to coat the wells are not specific to the antigen and bind many serum proteins
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