What is likely to be the problem if after you add substrate, all the wellsbegin to change to a deep color, including your controls? You may choosemore than onethe washing was not sufficientthe antibodies used to coat the wells are not binding the antigenO you may not have blocked the wells appropriatelyO the antibodies used to coat the wells are not specific to the antigen and bindmany serum proteins

ELISA is the technique in which antigens are immobilized in the well of a microtiter plate. An…

What is likely to be the problem if after you add substrate, all the wells begin to change to a deep color, including your controls? You may choose more than one Othe washing was not sufficient O the antibodies used to coat the wells are not binding the antigen O you may not have blocked the wells appropriately O the antibodies used to coat the wells are not specific to the antigen and bind many serum proteins

What is likely to be the problem if after you add substrate, all the wells begin to change to a deep color, including your controls? You may choose more than one Othe washing was not sufficient O the antibodies used to coat the wells are not binding the antigen O you may not have blocked the wells appropriately O the antibodies used to coat the wells are not specific to the antigen and bind many serum proteins

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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