What happens to beams of light from planes out of the focal plane in a confocal microscope? A.) They are blocked and absorbed by the dichroic mirror. B.) They are never emitted since the confocal microscope prevents off-focus fluorescence emission. C.) They reach the detector but are then removed by the computer from the measurement of planes at several heights. D.) They hit the pinhole without reaching the detector.
What happens to beams of light from planes out of the focal plane in a confocal microscope?
A.) They are blocked and absorbed by the dichroic mirror.
B.) They are never emitted since the confocal microscope prevents off-focus fluorescence emission.
C.) They reach the detector but are then removed by the computer from the measurement of planes at several heights.
D.) They hit the pinhole without reaching the detector.
The light from planes out of the focal plane in a confocal microscope.
The light of beam must be passes through the dichroic and is focused onto the pinhole.
The light of beam, that passes through the pinhole is measured by a detector.
The detector is joined with a computer which is Create the Photo.
Which Photo, created as only one pixel at a time.
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