Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.8: Normal Blood Cell Morphology
Problem 2RQ
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What does autoradiography reveal about cells actively expressing genes and what does this indicate?
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- Autoradiography is the technique that uses radiolabelling of the molecules for visualizing the samples.
- The techniques involve isolation of cellular components like DNA, RNA, proteins, or lipids followed by labeling with relevant isotopes.
- The principle of autoradiography is the precipitation of silver (Ag) atom that occurs due to the ionization of silver halide (Ag-X)
- The Ag-X molecule is encapsulated in gelatin that functions as a detector of decay from a radiolabeled sample.
- As the radioactive particle interacts with gelatin, AgX gets reduced and forms insoluble silver crystals.
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