Homework Help is Here – Start Your Trial Now!
Science
Biology
What can be concluded from the figure below?

What can be concluded from the figure below?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

What can be concluded from the figure below?

### Fig 4. Interactions between AphA, CRP, and the *cqsS* regulatory region. #### Panel (a) This panel displays ChIP-seq data indicating that AphA binds upstream of *cqsS* in vivo. Genes are represented as block arrows. The ChIP-seq coverage plots are provided for individual replicates, with AphA data in teal and control profiles in grey. Signals above or below the line correspond to reading mapping to the top or bottom strand. #### Panel (b) This illustrates the sequence of the *cqsS* intergenic region, with coding DNA in blue. The promoter elements, highlighted with underlines, identify the transcription start site with a bent arrow. The AphA binding sites are shown in boxes, the CRP binding site is marked in orange, and mutations at each AphA site are in red above the sequence. #### Panel (c) A DNase I footprinting assay using DNA containing PcqsS shows the binding of AphA and CRP. The gel uses a Maxam-Gilbert ‘G+A’ ladder for calibration. The addition of CRP at 3 μM results in a distinctive footprint (yellow bar). #### Panel (d) Electrophoretic mobility shift assay results on a DNA fragment containing PcqsS. AphA and CRP bind simultaneously at varying concentrations (lanes 2-6). #### Panel (e) This panel shows another electrophoretic mobility shift assay involving other DNA fragments, illustrating binding positions relative to *cqsS* and RNAI. #### Panel (f) Assay results demonstrate open complex formation when the RNA polymerase (RNAP), AphA, and CRP are present, further identifying shifts in the lane. #### Panel (g) A graph measuring expression in Miller units using a lacZ reporter fusion to PcqsS, showing differences in expression between WT and ΔaphA over optical density (650 nm). #### Panel (h) A schematic model summarizing findings: CRP and AphA binding on PcqsS leads to cqsS activation (ON state), while RNAP without them shows cqsS repression (OFF state). --- This image provides a detailed insight into the molecular interactions regulating the expression of *cqsS*, highlighting the roles of AphA and CRP in gene regulation.
Transcribed Image Text:### Fig 4. Interactions between AphA, CRP, and the *cqsS* regulatory region. #### Panel (a) This panel displays ChIP-seq data indicating that AphA binds upstream of *cqsS* in vivo. Genes are represented as block arrows. The ChIP-seq coverage plots are provided for individual replicates, with AphA data in teal and control profiles in grey. Signals above or below the line correspond to reading mapping to the top or bottom strand. #### Panel (b) This illustrates the sequence of the *cqsS* intergenic region, with coding DNA in blue. The promoter elements, highlighted with underlines, identify the transcription start site with a bent arrow. The AphA binding sites are shown in boxes, the CRP binding site is marked in orange, and mutations at each AphA site are in red above the sequence. #### Panel (c) A DNase I footprinting assay using DNA containing PcqsS shows the binding of AphA and CRP. The gel uses a Maxam-Gilbert ‘G+A’ ladder for calibration. The addition of CRP at 3 μM results in a distinctive footprint (yellow bar). #### Panel (d) Electrophoretic mobility shift assay results on a DNA fragment containing PcqsS. AphA and CRP bind simultaneously at varying concentrations (lanes 2-6). #### Panel (e) This panel shows another electrophoretic mobility shift assay involving other DNA fragments, illustrating binding positions relative to *cqsS* and RNAI. #### Panel (f) Assay results demonstrate open complex formation when the RNA polymerase (RNAP), AphA, and CRP are present, further identifying shifts in the lane. #### Panel (g) A graph measuring expression in Miller units using a lacZ reporter fusion to PcqsS, showing differences in expression between WT and ΔaphA over optical density (650 nm). #### Panel (h) A schematic model summarizing findings: CRP and AphA binding on PcqsS leads to cqsS activation (ON state), while RNAP without them shows cqsS repression (OFF state). --- This image provides a detailed insight into the molecular interactions regulating the expression of *cqsS*, highlighting the roles of AphA and CRP in gene regulation.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Dissection
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS