What are the two types of ELISA methods and how do they work? What is a chromogen?
Enzyme-linked immunosorbent assay (ELISA) is a non-isotopic immunoassay. An enzyme is utilized as a name in ELISA instead of radioactive isotope utilized in RIA. ELISA is pretty much as sensitive as or significantly more sensitive than RIA. There is no danger of radiation risks (similar to the case with RIA) in ELISA.
ELISA depends on the immunochemical standards of antigen-antibody reaction.
ELISA is broadly utilized for the assurance of little amounts of proteins (chemicals, antigens, antibodies) and other organic substances. The most usually utilized pregnancy test for the discovery of human chorionic gonadotropin (hCG) in urine depends on ELISA. By this test, pregnancy can be identified inside a couple of days later origination. ELISA is likewise been utilized for the conclusion of AIDS. The diagrammatic representation of ELISA is here:
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