Q: Describe the principle of ELISA
A: (Since what is IF is not clearly mentioned question; thus we have answered ELISA principle below.)…
Q: What are different methods used to generate vaccines?
A: Vaccines are used artificially to induce an immune response. By using the vaccine, antibodies…
Q: Explain how labeled antibodies are used in direct and indirect tests.
A: Antibodies or immunoglobulins are the primary line of defense in the body. They recognize and bind…
Q: Define the Recommended Immunization Schedule for Adults ?
A: Immunization or vaccination is a preventive health strategy that protects from disease by…
Q: What is Immunoprecipitation?
A: Immunoprecipitation is a technique that is used to isolate a specific protein complex from a…
Q: Explain what immunoaffinity extraction consists of when applied to obtaining steroids
A: Liquid chromatography (LC) and the targeted attachment of antibodies or related substances are both…
Q: What causes primary immunodefi ciencies and acquired immunodefi ciencies?
A: Immunodeficiency, otherwise called immuno-compromisation, is a state where the immune framework's…
Q: Why do you coat the elisa plate with albumin?
A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…
Q: What is the purpose of anti-human IgG antibodies in immunological testing?
A: Each IgG is composed of four peptide chains with two antigen binding sites - two γ-class heavy…
Q: What are the four steps of an Elisa protocol
A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure…
Q: what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the…
A: ELISA=Enzyme linked immunosorbent assay.
Q: What are five commercially available immunodiagnostic kits?
A: Five commercially available immunodiagnostic kits are as follows : 1)HIV CheX: It is an ELISA pack…
Q: Briefl y describe the principles and give an example of the use of a specifi c test using…
A: There are several diseases that affect humans and timely detection of these diseases can help in…
Q: Describe the process of immunohistochemistry to detect a specific protein of interest.
A: or ImmunoHistoChemistry ( IHC), is an uncommon staining measure performed on new or frozen breast…
Q: Explain the differences between direct and indirect procedures inimmunoassay tests, giving examples.
A: Immunoassays are biochemical test based on antigen-antibody interactions. It is used to determine…
Q: Describe and give one example: artificial active acquired immunity artificial passive…
A: Artificial active acquired immunity: A protection produced by intentional…
Q: Describe the principles behind direct and indirect fl uorescent antibody tests.
A: Fluorescent antibody technique is a laboratory technique in which the labelled antibodies bind to…
Q: Describe the term serology and explain the immunologic principle behind serological tests.
A: Immune reactions to antigens result in the production of antibodies, and antibodies strongly attach…
Q: What are natural activeimmunization and artificialactive immunization?
A: Immunity refers to every mechanism of the body that helps to protect the body from harmful foreign…
Q: Interpret the outcome of direct and indirect immunofl uorescent antibody testing.
A: Antigen or Antibody detection can be done nowadays in many different ways. Enzyme-linked…
Q: What are the different applications of ELISA?
A: ELISA is the basic assay technique, known as enzyme-linked immunosorbent assay (also referred to as…
Q: Describe the method, time frame, and rationale for the tuberculinskin test. What component of the…
A: The tuberculin skin test is also known as Mendel–Mantoux test or Mantoux test. It is a tool used for…
Q: What are advantages and disadvantages of the antibody test with the coronavirus?
A: We know that The Severe Acute Respiratory Syndrome corona virus-2 (SARS-CoV-2) is responsible for…
Q: What is the immunoassay analyzer Machine
A: Immunoassay is a test that is used to measure the concentration of a macromolecule or a small…
Q: What is the geometry of molecules of the three dimensional shape of molecules in immunoassay methods…
A: Shape is an important molecular feature determining the fate of a compound. It does so in terms of…
Q: Describe an ELISA test to detect the presence of HIV antibodies in a patient.
A: HIV is a virus than can cause AIDS if not treated .ELISA is the one of the test to screen HIV .…
Q: Define the term passive immunization?
A: Immunization is a process in which an individual's immune system is boosted against an infectious…
Q: Identify two immunological diagnostic techniques that rely on a secondary antibody, and explain how…
A: Serology is the scientific study of blood serum and other bodily fluids. In practical immunological…
Q: Compare the advantages and disadvantages of EIA, rapid tests,and immunoblots with respect to speed,…
A: The immune system is a complex network of biological structures (cell) and protein that defends the…
Q: In general, How do Primary immunodeficiencies and acquired immunodeficienciesdiffer in their basic…
A: When the Immune system fails to protect the host from the disease-causing organisms or from abnormal…
Q: What are the types of Vaccines?
A: A vaccination is a biological preparation that gives people active acquired immunity against a…
Q: t Are The Uses Of Indirect Immuno Fluorescence?
A: The immunofluorescence technique, which used a fluorescence microscope to read the specific…
Q: Define the term serology, and explain the immunologic principle behind serological tests.
A: An antigen is a substance or a molecule that the body recognizes as a foreign particle. That means…
Q: Provide some reasons in vivo tests would not be as reliable as immunoassaytests.
A: IMMUNOASSAY test can be used for the presence of specific antibody or specific antigen in the blood…
Q: What is passive immunisation?
A: Immunity is the ability of an organism to fight with the diseases. Any foreign organism or particle…
Q: Briefly describe the principles and give an example of the use of aspecific test using…
A: these immunological techniques focus on identifying the antigens [mostly proteins] or antibodies in…
Q: Why are immunosuppressed individuals given antifungal agents?
A: 'Immunocompromised' refers to a weak immune system that is not functioning properly.
Q: What is the effect of not adding the antigen to the ELISA reaction?
A: ELISA stands for Enzyme Linked Immunosorbent assay. This technique is based on antigen antibody…
Q: In ELISA, what is the importance of washing? When does washing is performed?
A: Introduction :- Enzyme-linked immunoassay (ELISA) is an acronym for enzyme-linked immunoassay.…
Q: Interpret the outcome of direct and indirect immunofluorescentantibody testing.
A: Fluorescent Antibody techniques are utilized for the rapid visualization of bacteria that involves…
Q: What are the two types of ELISA methods and how do they work? What is a chromogen?
A: Enzyme-linked immunosorbent assay (ELISA) is a non-isotopic immunoassay. An enzyme is utilized as a…
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- Describe the term serology and explain the immunologic principle behind serological tests.What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?What are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.
- What is the principle of Immunochromatographic system, and its uses? Long anwserIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.In an immunoassay what is a secondary antibody? Antibodies used to recognize antigens. They confer specificity to the immunoassay. Secondary antibodies line the wells of the ELISA test strip. Secondary antibodies recognize and bind to primary antibodies. Secondary antibodies interact directly with the antigen.