Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Explain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram…
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…
A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…
Q: Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the…
A: Question - Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…
A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…
Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…
A: The microorganisms that can be found in several areas of the habitat and come under the category of…
Q: In a Gram-stained smear, medium-sized rods with rounded, pink ends were found, arranged chaotically.…
A: The Gram stain is a widely used method for differentiating between different types of bacteria. The…
Q: Which of the following can be used to distinguish one colony from another on an agar plate? Select…
A: A colony is a visible cluster of microorganisms like fungi and bacteria that grow on a solid growth…
Q: the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell…
A: A mordant or color fixative is defined as a substance used to set (for example tie) colors on…
Q: 3. What information can be obtained from a simple positive staining procedure? 4. What is the…
A: Gram staining is a popular method for distinguishing between two broad groups of bacteria based on…
Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).
A: The danish Bacteriologist Christian gram discovered this differential technique in order to…
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…
A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: 4. The Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the…
A: Decoloriser in gram staining method is to dehydrate the peptidoglycan layer of the cell wall causing…
Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…
A: Microscopy is the technique which is used to magnify small microorganisms and small specimens . It…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: This part of the staining process helps the primary stain to remain in the cell during…
A: Staining of biological samples is a very useful process for detailed observation and measurement of…
Q: Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image…
A: Bacteria can be classified into two different classes on the basis of the staining method:…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: Give two factors that may affect a good gram staining technique output. Provide an explanation how…
A: Many factors can affect the results of gram staining. However, two important factors that may affect…
Q: Explain how you would troubleshoot a Gram stain of a known G(-) organism that stained purple.
A: Gram staining is used to differentiate bacteria based on their cell wall constituents. Gram-positive…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…
A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…
Q: The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain…
A: Bacillus anthracis is a Gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease…
Q: The GRAM stain method was applied on both pictures. What is the result found on each of them? A A.
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram…
What are the expected result after
a. Crystal Violet =
b. Gram Iodine =
c. Ethanol =
d. Safranin =
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- Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingWhich of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C. Ethanol D. Crystal Violetthe purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stain
- The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. SafranineGive two factors that may affect a good gram staining technique output. Provide an explanation how these factors influence the result.Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the cytoplasm of the cell walls C. Can be used to stain peptidoglycan on bacteria cell wall D. Can be used as an effective antimicrobial
- Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few secondsExplain the principle of Gram staining. (You do not need to describe the procedure step by step).The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method
- 3. What information can be obtained from a simple positive staining procedure? 4. What is the difference between a simple and differential stain? 5. What is the reagent and purpose for each of the following gram stain steps? a. Primary stain b. Mordant c. Decolorizer d. Counterstainobserve the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the aboveExplain the different results in the Gram stain are due to differences in the structure of the cell wall and how it reacts to the series of reagents applied to the cells.