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volts should my gel be run

volts should my gel be run

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Need question 5 answer on how many volts should my gel be run
3. Ligation: Volume to Add Added? Reaction Component to Reaction Insert: huma genomic EcoRI/Hindlll-digested pUC19 plasmid (20 ng/µl) Need 80 ng 4 MI Vector: EcoRI/Hindlll-digested pUC19 plasmid (20_ng/µl) Need 25 ng 1.25 Ml 20M! 25 Ligase enzyme レ (lo x ) Ligase buffer 2MI Water 11.75MI Total Volume 20 μι CIVI = ezvz 4. Gel electrophoresis: Amount of DNA Ladder to add to gel (Lane 1): 5u t Iml u 10adin +4Pe Volume of Volume of Sample Sample Total Volume Sample Loading Dye Undigested human 9DNA Lane 2 5 ul Lane 3 Digested human gDNA 5 μι IMI しu Undigested pUC19 plasmid Lane 4 5 µl IMI Digested pUC19 plasmid Lane 5 5 µl しM」 5. Measure the distance between electrode wires in the bottom of the gel box. At how many volts should you run your gel? (see p. 64 2b.) 13.5 cm olait
Transcribed Image Text:3. Ligation: Volume to Add Added? Reaction Component to Reaction Insert: huma genomic EcoRI/Hindlll-digested pUC19 plasmid (20 ng/µl) Need 80 ng 4 MI Vector: EcoRI/Hindlll-digested pUC19 plasmid (20_ng/µl) Need 25 ng 1.25 Ml 20M! 25 Ligase enzyme レ (lo x ) Ligase buffer 2MI Water 11.75MI Total Volume 20 μι CIVI = ezvz 4. Gel electrophoresis: Amount of DNA Ladder to add to gel (Lane 1): 5u t Iml u 10adin +4Pe Volume of Volume of Sample Sample Total Volume Sample Loading Dye Undigested human 9DNA Lane 2 5 ul Lane 3 Digested human gDNA 5 μι IMI しu Undigested pUC19 plasmid Lane 4 5 µl IMI Digested pUC19 plasmid Lane 5 5 µl しM」 5. Measure the distance between electrode wires in the bottom of the gel box. At how many volts should you run your gel? (see p. 64 2b.) 13.5 cm olait
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